Publication Date

Fall 2014

Document Type

Project Summary

Degree Name

Master of Science

Department

Analytical Chemistry

First Advisor

Joong-Won Shin, Ph.D.

Second Advisor

Walter Henne, Jr., Ph.D.

Third Advisor

Kulugammana Ranmohotti, Ph.D.

Abstract

Chapter 1:

The aim of this research project was to measure the differences in proton affinities of tyrosine and its halogenated forms, 3-chlorotyrosine and 3-iodotyrosine. Tandem mass spectrometry was used here to measure the proton affinities. These amino acids were formed as protonated dimers with amino acids of known proton affinity and were introduced into the instrument through electrospray ionization (ESI). Based on the intensities of the protonated amino acids appeared in the mass spectrum when the dimer was isolated and fragmented, the proton affinities of the sample amino acids were calculated.

Chapter 2:

The research project was on mass spectrometry of mixtures of peptides and metals. A tripeptide was mixed with different metals of certain concentration and were introduced into the instrument through electrospray ionization (ESI) using a syringe pump. Later, the [metal+peptide-H]+ of the tripeptide and the metal ion was isolated and fragmented. This shows how the tripeptide fragments specific to each of the doubly charged metal ion.

Available for download on Sunday, April 01, 2018

Share

COinS