Master of Science
Walter Henne, Jr., Ph.D.
Timothy Gsell, Ph.D.
Aheda Saber, Ph.D.
The main objective of my project is to determine the bacterial cell wall intermediates in E.coli and develop an LCMS method that Phosphomycin is a PEP analogue that irreversibly inhibits UDP-GluNAc-enolpyruvate transferase (MurA enzyme), which prevents the formation of N-acetyl muramic acid, which is an essential element in the Peptidoglycan cell wall. Antibacterial agents have a tremendous impact on human health. Most of the antibacterial agents, including beta lactams target the bacterial cell wall biosynthesis.The pathway for Escherichia coli is shown in page 19. Among the most widely used antibiotics, beta lactams inhibit the penicillin binding protein (PBPs)which block the cross linking reactions of cell wall biosynthesis. A Liquid chromatography-tandem mass spectrometry provides a new technology to detect and quantitation of cell wall intermediates.
Arigapudi, Chandra Sekhar, "An LCMS Based Assay for Detection and Quantification of Bacterial Cell Wall Intermediates" (2011). All Capstone Projects. 12.