Publication Date

Fall 2010

Document Type

Project Summary

Degree Name

Master of Science


Analytical Chemistry

First Advisor

Patty Fu-Giles, Ph.D.

Second Advisor

Karen D'Arcy, Ph.D.

Third Advisor

Walter Henne, Jr., Ph.D.


Absorption and fluorescence emission spectra of Carminic acid were recorded in solvents with different solvent parameters like polarities, dielectric constant and refractive index. The solvents used in the experiment were water, DMSO, acetonitrile, methanol, propanol and butanol. The atomic radius of the molecule was found out using Hartree-Fock method in molecular modeling using Spartan software and it is applied to find out the dipole moments. The dipole moments of Carminic acid in the ground state and excited states have been determined and Solvatochromic shift method has been used to determine the excited state dipole moments. The linear progressions were made and the slopes were determined from absorbance and fluorescence band shifts. The conclusions on the spectral shifts were done based on the polarity function of the solvents and the dipole moments in ground and excited states of the molecule.