Comparing the Colony Formation of Pseudomonas aeruginosa on Daily and Monthly Contact Lenses with Different Oxygen Permeability
Type of Presentation
Poster Session
Location
D2400 - University Library
Start Date
4-17-2025 11:30 AM
End Date
4-17-2025 12:45 PM
Abstract
Bacterial presence and microbial formation on contact lens can cause serious corneal infection including keratitis. There are many bacterial prevention methods available today, however, the chances of bacterial growth on contact lenses are still high. The best way to prevent bacterial growth on contact lenses is by practicing proper hygiene and caring when handling contact lenses. The main objective of this study is to compare the number of colonies on the oldest and newest versions of daily contact lenses versus oldest and newest versions of monthly contact lenses. The type of microorganism that was used in this study was Pseudomonas aeruginosa. The daily contact lenses that were used in the study include 1-Day Acuvue Moist which is one the oldest and Precision 1, which is one of the newest versions of the daily contact lenses. The monthly contact lenses that were used in the study include Biofinity energys which is one of the oldest and Total 30 which is one of the newest versions of the monthly contact lenses. The research was done in the microbiology lab at Governors State University. 5 contact lenses from each brand were the control groups. It was placed in sterile tubes with 5 ml of TSB broth but without Pseudomonas aeruginosa culture. 5 contact lenses from each brand were placed in sterile tubes with 5mL TSB broth along with Pseudomonas aeruginosa culture in an incubator at 37 degrees Celsius for 24 hours. After 24 hours, all test tubes were taken out from the incubator. Swabbed the bacteria from the contact lenses and did a spread plate which was then placed in the incubator for 24 hours at 37 degrees Celsius. After 24 hours, since the colonies were too many to count, performed a 1:1000, and 1:10,000 serial dilutions. Colonies were high on the daily contact lenses, specifically the 1-day Acuvue moist. Therefore, contact lenses with lower oxygen permeability had more bacterial growth compared to contact lenses with higher oxygen permeability.
Faculty / Staff Sponsor
Dr. Timothy Gsell
Comparing the Colony Formation of Pseudomonas aeruginosa on Daily and Monthly Contact Lenses with Different Oxygen Permeability
D2400 - University Library
Bacterial presence and microbial formation on contact lens can cause serious corneal infection including keratitis. There are many bacterial prevention methods available today, however, the chances of bacterial growth on contact lenses are still high. The best way to prevent bacterial growth on contact lenses is by practicing proper hygiene and caring when handling contact lenses. The main objective of this study is to compare the number of colonies on the oldest and newest versions of daily contact lenses versus oldest and newest versions of monthly contact lenses. The type of microorganism that was used in this study was Pseudomonas aeruginosa. The daily contact lenses that were used in the study include 1-Day Acuvue Moist which is one the oldest and Precision 1, which is one of the newest versions of the daily contact lenses. The monthly contact lenses that were used in the study include Biofinity energys which is one of the oldest and Total 30 which is one of the newest versions of the monthly contact lenses. The research was done in the microbiology lab at Governors State University. 5 contact lenses from each brand were the control groups. It was placed in sterile tubes with 5 ml of TSB broth but without Pseudomonas aeruginosa culture. 5 contact lenses from each brand were placed in sterile tubes with 5mL TSB broth along with Pseudomonas aeruginosa culture in an incubator at 37 degrees Celsius for 24 hours. After 24 hours, all test tubes were taken out from the incubator. Swabbed the bacteria from the contact lenses and did a spread plate which was then placed in the incubator for 24 hours at 37 degrees Celsius. After 24 hours, since the colonies were too many to count, performed a 1:1000, and 1:10,000 serial dilutions. Colonies were high on the daily contact lenses, specifically the 1-day Acuvue moist. Therefore, contact lenses with lower oxygen permeability had more bacterial growth compared to contact lenses with higher oxygen permeability.