Publication Date

Spring 2012

Document Type


Degree Name

Master of Science


Analytical Chemistry

First Advisor

Patty Fu-Giles, Ph.D.

Second Advisor

Walter Henne, Jr., Ph.D.

Third Advisor

Karen D'Arcy, Ph.D.


Humic acid (HA) are widely dispersed, naturally occurring biopolymers most commonly found in soil, drinking water, and plants. Humic acid is soluble in alkali and insoluble in an acidic medium or water. In the present study the interaction of Humic acid with DNA is determined by the calculation of the binding constant of humic acid with CT-DNA and the quenching phenomenon of humic acid in the presence of DNA is also studied using Stern Volmer Relationship.