Capture and Analysis of Folate Receptor Positive Cells using Magnetic Beads
Type of Presentation
Event
Location
Hall of Governors
Start Date
4-1-2016 1:00 PM
End Date
4-1-2016 3:00 PM
Abstract
Folate receptor alpha is a membrane-bound protein displaying high affinity for folic acid. This receptor serves as a receptor-mediated transport system of folic acid into cancer and cells associated with inflammation (e.g., macrophages). Folate is a basic component of cell metabolism in both synthesis of DNA and proteins, which is hypothesized to be necessary for maintaining adequate processes during increased metabolic demand. Interestingly, most normal cells in the body have little or no high affinity folate receptor alpha. Based on these attributes, folate based drug delivery, imaging systems, and diagnostic systems are in several stages of development worldwide. In this project, capture, detection and analysis of FR+ cells will be undertaken using a novel folate PEG 6-Histidine conjugate that was recently synthesized in the Henne research lab. This conjugate is capable of binding to NTA-Ni in the classic 6-His capture systems including NTA-Ni magnetic beads and Nano composites. Numerous methods have been explored in the literature in an attempt to detect and capture low levels of folate positive cells including rare circulating cancer cells in circulation including QCM biosensors, SPR biosensors, and immuno-magnetic diffraction detection. To our knowledge, this is the first time a folate 6-His conjugate has been used in this fashion.
Presentation File
wf_yes
Capture and Analysis of Folate Receptor Positive Cells using Magnetic Beads
Hall of Governors
Folate receptor alpha is a membrane-bound protein displaying high affinity for folic acid. This receptor serves as a receptor-mediated transport system of folic acid into cancer and cells associated with inflammation (e.g., macrophages). Folate is a basic component of cell metabolism in both synthesis of DNA and proteins, which is hypothesized to be necessary for maintaining adequate processes during increased metabolic demand. Interestingly, most normal cells in the body have little or no high affinity folate receptor alpha. Based on these attributes, folate based drug delivery, imaging systems, and diagnostic systems are in several stages of development worldwide. In this project, capture, detection and analysis of FR+ cells will be undertaken using a novel folate PEG 6-Histidine conjugate that was recently synthesized in the Henne research lab. This conjugate is capable of binding to NTA-Ni in the classic 6-His capture systems including NTA-Ni magnetic beads and Nano composites. Numerous methods have been explored in the literature in an attempt to detect and capture low levels of folate positive cells including rare circulating cancer cells in circulation including QCM biosensors, SPR biosensors, and immuno-magnetic diffraction detection. To our knowledge, this is the first time a folate 6-His conjugate has been used in this fashion.
Other Presentation Disciplines:
Dr. Walter Henne is an Associate Professor of Chemistry and Mr. Sean Dwyer is a graduate student in Analytical Chemistry in the College of Arts and Sciences.