Event Title

What Happens with Bacteria When They Get Killed?

Location

Hall of Governors

Start Date

1-4-2016 10:00 AM

End Date

1-4-2016 12:00 PM

Description

Detection of volatile components over Escherichia coli at full growth and after treatment with heat, UV light and antibacterial bleach using GC/MS with solid-phase microextraction (SPME). Bacteria are known to produce a range of volatile organic compounds (VOCs). VOCs are known as products or by-products of metabolic process that occurs in bacteria. This includes the generation of hydrocarbons, aliphatic alcohols and ketones from fatty acid biosynthesis and break down of the amino acid tryptophan. Many of VOCs can be detected with human nose can be apply as specific smell of certain bacteria that prove it presence. The research has shown that using GC-MS with head space solid-phase microextraction (HS-SPME) is a good tool for detection of bacteria volatile organic compounds. Proposed method in this paper is focusing on detection of VOCs produced by bacteria when get killed, in other word what happens with bacteria when get killed. The experiment will be performed using GC-MS with HS-SPME over Escherichia coli. Experiment will be done of four steps. We will need to prepare four cultures of E. Coli at the same time and under the same conditions. a) will be used to detect and validate volatile components at full growth, b) we will apply heat and then examine volatile components, c) will treat with UV light and last one d) with antibacterial bleach and do examination. After experiment is done all data will be collected and treated bacteria results will be compare with untreated E. Coli.

Comments

Dr. John Sowa is the Chair, Division of Science and Mr. Veroljub Velickovic is an undergraduate student in Chemistry in the College of Arts and Sciences.

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Apr 1st, 10:00 AM Apr 1st, 12:00 PM

What Happens with Bacteria When They Get Killed?

Hall of Governors

Detection of volatile components over Escherichia coli at full growth and after treatment with heat, UV light and antibacterial bleach using GC/MS with solid-phase microextraction (SPME). Bacteria are known to produce a range of volatile organic compounds (VOCs). VOCs are known as products or by-products of metabolic process that occurs in bacteria. This includes the generation of hydrocarbons, aliphatic alcohols and ketones from fatty acid biosynthesis and break down of the amino acid tryptophan. Many of VOCs can be detected with human nose can be apply as specific smell of certain bacteria that prove it presence. The research has shown that using GC-MS with head space solid-phase microextraction (HS-SPME) is a good tool for detection of bacteria volatile organic compounds. Proposed method in this paper is focusing on detection of VOCs produced by bacteria when get killed, in other word what happens with bacteria when get killed. The experiment will be performed using GC-MS with HS-SPME over Escherichia coli. Experiment will be done of four steps. We will need to prepare four cultures of E. Coli at the same time and under the same conditions. a) will be used to detect and validate volatile components at full growth, b) we will apply heat and then examine volatile components, c) will treat with UV light and last one d) with antibacterial bleach and do examination. After experiment is done all data will be collected and treated bacteria results will be compare with untreated E. Coli.