Master of Science
K. G. Sanjaya Ranmohotti, Ph.D.
Jose Perez, Ed.D.
Kerri Morris, Ph.D.
A compound was specifically designed to further aid research on the detection and treatment of folate receptor positive cancer cells. A small but complex peptide chain was commercially produced to have a flexible glycine spacer and a six-histidine tag. Histidine tagging was chosen for its ability to bind to nickel. The compound in this report was purified and analyzed by HPLC and confirmed via mass spectrometry. This binding capacity and the ability of folate conjugates to bind to cancer cell receptors have both been explored for the past quarter of a century [1-3]. Folate conjugates have been successfully used as a transporter of cytotoxins and imaging agents through folate receptors (FRs), while histidine tagging has been used for imaging with dyes, separation, and purification with columns and metallic beads [4-14].
Kampner, Alexander, "Synthesis and Analysis of Folate Histidine" (2017). All Capstone Projects. 326.