Publication Date

Summer 2017

Document Type

Project Summary

Degree Name

Master of Science


Analytical Chemistry

First Advisor

K. G. Sanjaya Ranmohotti, Ph.D.


The purpose of this study is to develop an analytical method for determining the concentration of dialkyl phthalates as part of a larger study of phthalate degrading bacteria. Specifically, bacteria isolated from polyaromatic hydrocarbon contaminated riverine sediment are being determined for their proficiency in degrading dimethyl phthalate, diethyl phthalate, and dibutyl phthalate." HPLC instrument was used for analysis of these phthalate derivatives. A reversed phase isocratic and gradient elution and UV detection method was used for the individual and simultaneous determination of these phthalate derivatives. HPLC was carried out using a C18 column and spectrophotometric detection at 230nm. An isocratic elution was performed using methanol–water (75/25 %) as a mobile phase. Standard calibration curves were linear for all three analytes.