Research Day 2018 Schedule

Event Title

Quantitative Analysis of Anti-histamine, Anti-diabetic, and Analgesic Pharmaceutical Drugs by High Performance Liquid Chromatography

Location

D1496

Start Date

4-6-2018 10:10 AM

End Date

4-6-2018 11:10 AM

Abstract

Three representative drugs, one each from anti-histamine, anti-diabetic, and analgesic categories were analyzed by High Performance Liquid Chromatography (HPLC). Cetirizine dihydrochloride, the active ingredient of anti-histamine drug, Zyrtec, was analyzed by HPLC using Grace C-18 column with 1 mL/minute flow of a mobile phase comprising a 30:70 mixture of 2.25 pH phosphate buffer solution and acetonitrile with a UV detector at 230 nm wavelength. A set of ten standard solutions, ran in triplicate, in the concentration range of 11 – 110 mg/mL yielded a linear curve with R2 value of 0.9994, thus establishing a working calibration curve in this range. Analysis of tablets from a commercial sample showed to contain 8.2 mg in each tablet, which is 18 % lower than the amount of 10 mg stated in the label of the product. In the category of anti-diabetic drugs, linagliptin, the active ingredient of Tradjenta, was analyzed by a similar method with a slight variation in the mobile phase and UV detector wavelength. A linear calibration curve with R2 value of 0.9997 was established for a concentration range of 5 – 50 mg/mL. In the category of analgesic drugs, ibuprofen, the active ingredient of Motrin, was analyzed by a similar method with a slight variation in the mobile phase and UV detector wavelength. A linear calibration curve with R2 value of 0.9994 was established for a concentration range of 0.21 – 1.9 mg/mL. The future studies include comparison of these results with analysis of these drugs by quantitative nuclear magnetic resonance (q-NMR) technique.

Identify Grant

1. Undergraduate Creative Activity, Research, and Scholarship (UCARS) Grant

2. Gift of linagliptin API sample from MSN Pharmaceuticals Inc., Edison, NJ

Faculty / Staff Sponsor

Dr. Shailendra Kumar

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Apr 6th, 10:10 AM Apr 6th, 11:10 AM

Quantitative Analysis of Anti-histamine, Anti-diabetic, and Analgesic Pharmaceutical Drugs by High Performance Liquid Chromatography

D1496

Three representative drugs, one each from anti-histamine, anti-diabetic, and analgesic categories were analyzed by High Performance Liquid Chromatography (HPLC). Cetirizine dihydrochloride, the active ingredient of anti-histamine drug, Zyrtec, was analyzed by HPLC using Grace C-18 column with 1 mL/minute flow of a mobile phase comprising a 30:70 mixture of 2.25 pH phosphate buffer solution and acetonitrile with a UV detector at 230 nm wavelength. A set of ten standard solutions, ran in triplicate, in the concentration range of 11 – 110 mg/mL yielded a linear curve with R2 value of 0.9994, thus establishing a working calibration curve in this range. Analysis of tablets from a commercial sample showed to contain 8.2 mg in each tablet, which is 18 % lower than the amount of 10 mg stated in the label of the product. In the category of anti-diabetic drugs, linagliptin, the active ingredient of Tradjenta, was analyzed by a similar method with a slight variation in the mobile phase and UV detector wavelength. A linear calibration curve with R2 value of 0.9997 was established for a concentration range of 5 – 50 mg/mL. In the category of analgesic drugs, ibuprofen, the active ingredient of Motrin, was analyzed by a similar method with a slight variation in the mobile phase and UV detector wavelength. A linear calibration curve with R2 value of 0.9994 was established for a concentration range of 0.21 – 1.9 mg/mL. The future studies include comparison of these results with analysis of these drugs by quantitative nuclear magnetic resonance (q-NMR) technique.