Publication Date

Summer 2017

Document Type

Project Summary

Degree Name

Master of Science

Department

Analytical Chemistry

First Advisor

John Sowa, Ph.D.

Second Advisor

Walter Henne, Ph.D.

Third Advisor

K. G. Sanjaya Rammohotti, Ph.D.

Abstract

An easy, efficient and safe method was developed to determine iron in water samples. The method is an Iron Cell Test kit from Spectroquant in which firstly all iron ions are reduced to iron (II) ions by ascorbic acid. In a thioglycolate buffered medium, iron (II) reacts with a triazine derivative to form a purple complex that is determined photometrically. Calibration curve of iron standards was done with concentrations of 0.50, 1.0, 2.0, 3.0 and 4.0 ppm and it gave a R2 value of 0.9989 and straight line equation y=0.4749x-0.046. Iron analysis was done on two sets of water samples. Named as set I samples 1, 2, 3, 4, 5, and 6 and set II samples 1, 2, 3, 4 and 5, they were acidified with 0.1% HNO3 and the absorbance was measured in a UV-Visible Spectrometer at 565 nm. The concentrations were found as 0.45, 0.13, 3.84, 5.64, 6.72, 5.78 ppm for set I samples and 0.11, 0.11, 0.14, 0.12, and 0.11 ppm, for set II samples respectively. The limit of detection (LOD) is 0.10 ppm, and, the limit of quantification (LOQ) is 1.0 ppm.

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