Evaluation and In Vitro Studies of Folate PEG-Biotin and Other Polyethylene Glycol Agents
Type of Presentation
Event
Location
Hall of Governors
Start Date
4-7-2017 12:30 PM
End Date
4-7-2017 1:30 PM
Abstract
In this literature thesis, the folate-fluorescein-PEG-biotin (FFPB) complex and ligand have been successfully characterized and purified. FFPB has been further incorporated into a scheme for secondary detection of positive cells that is detected by folate receptors using fluoresence microscopy. However, high affinity for folic acid has been displayed by the membrane bound folate receptor alpha protein. Folic acid receptor-facilitated transport system is believed to act in cancer cells as well as inflammatory associated cells. It is interesting to know that majority of the normal body cells have little affinity for folic acid when there is no high affinity alpha folate receptor presence. Based on these features, diagnostic systems, folate dependent drug delivery and imaging systems are in more than a few stages of investigation worldwide. Various methods have been reported in the literature to evaluate minimum levels of free folate receptor in circulating cancer cells. It may be possible to take advantage of these types of systems, using registered folate-fluorescein-PEG-biotin captured ligand that is manufactured by Hussain, Syed (2012). This literature report reviews the current use of a previously synthesized folate PEG probe. In this report, further purification and characterization has been carried out of a folate based biotin probe, which is eventually incorporated into a detection scheme. Using this method essentially prevents false positive incidents resulting from capture of non-targeted cells and non-specific binding. Eventually, the results of the study reveal that the utilization of PEG as a spacer has been accounted to re-establish a high affinity between biotin subsidiaries with avidin.
Evaluation and In Vitro Studies of Folate PEG-Biotin and Other Polyethylene Glycol Agents
Hall of Governors
In this literature thesis, the folate-fluorescein-PEG-biotin (FFPB) complex and ligand have been successfully characterized and purified. FFPB has been further incorporated into a scheme for secondary detection of positive cells that is detected by folate receptors using fluoresence microscopy. However, high affinity for folic acid has been displayed by the membrane bound folate receptor alpha protein. Folic acid receptor-facilitated transport system is believed to act in cancer cells as well as inflammatory associated cells. It is interesting to know that majority of the normal body cells have little affinity for folic acid when there is no high affinity alpha folate receptor presence. Based on these features, diagnostic systems, folate dependent drug delivery and imaging systems are in more than a few stages of investigation worldwide. Various methods have been reported in the literature to evaluate minimum levels of free folate receptor in circulating cancer cells. It may be possible to take advantage of these types of systems, using registered folate-fluorescein-PEG-biotin captured ligand that is manufactured by Hussain, Syed (2012). This literature report reviews the current use of a previously synthesized folate PEG probe. In this report, further purification and characterization has been carried out of a folate based biotin probe, which is eventually incorporated into a detection scheme. Using this method essentially prevents false positive incidents resulting from capture of non-targeted cells and non-specific binding. Eventually, the results of the study reveal that the utilization of PEG as a spacer has been accounted to re-establish a high affinity between biotin subsidiaries with avidin.